Medicinal plants hold a significant place as alternative treatments available for inflammatory\ndiseases, with many phytoconstituents being frequently tested in vitro for their biological activities.\nIn the current study, we investigated the in vivo anti-inflammatory properties of a novel active gel\nformulation, combining Achillea millefolium and Taxodium distichum essential oils with extracts of\nAesculus hippocastanum seeds and Plantago lanceolata leaves. The toxicity of the obtained extracts and\nvolatile oils was determined using the invertebrate model based on Daphnia magna. Anti-inflammatory\npotential was evaluated by the plethysmometric method onWistar rats, expressed as the inhibition of\nthe inflammatory oedema (%IIO), while the antinociceptive response was determined on NMRI mice,\naccording to the tail-flick latency method. The tested gelâ??s ecacy was similar to the 5% diclofenac\nstandard (maximal %IIO of 42.01% vs. 48.70%, respectively), with the anti-inflammatory eect being\nobserved sooner than for diclofenac. Our active gel also produced a significant prolongation of\ntail-flick latencies at both 60 and 120 min, comparable to diclofenac. Consequently, we can imply that\nthe active constituents present in vivo anti-inflammatory properties, and the prepared gel may be\nsuited for use as an alternative treatment of topical inflammatory conditions.
Loading....